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简要描述:NCI-H1693细胞;人非小细胞肺癌细胞传代:如果细胞密度达80%-90%,即可进行传代培养。细胞名称:NCI-H1693 人非小细胞肺癌细胞组织来源:肺培养条件:1640 +10% FBS形 态:贴壁;上皮细胞样规 格:1×10^6 cells/瓶
产品型号:
所属分类:人细胞系
更新时间:2016-11-07
NCI-H1693细胞;人非小细胞肺癌细现货供应,代次低,状态好,现货。
规格:70%密度的25cm2培养瓶的细胞一瓶
包装:内层无菌自封袋;防压泡沫盒;外层防压保温气泡袋;说明书
培养条件:DMEM +10% FBS
细胞数量:1× 106个
细胞传代:1:4~1:6传代;每周换液2~3次
问题一:NCI-H1693细胞;人非小细胞肺癌细长得很快很好,30万一瓶,3天就长满,也没出现污染,死细胞也较少。但是消化细胞后,用培养基中和消化液,在细胞计数板上可以看见细胞粘成一小团团的,无法准确计数,很影响种板的准确性,但传代后细胞铺板均匀,生长得也很好。我尝试了很多次细胞计数,每次都出现细胞成团的问题。
问题解答1:遇到这种情况,办法有两个,1.就是不能等到NCI-H1693细胞;人非小细胞肺癌细长得很满时才去消化计数,可以在密度有90%时就去计数,如果怕细胞量不够铺板可以两瓶甚至三瓶合在一起。2.如果聚团还是很严重,我就把一个细胞团默认为3-4个细胞这样来计数,发现这样计数跟实际细胞数也没有太大出入,之后我就一直这样做了。我查了很多资料,都说这个细胞容易抱团生长,因为有突触,所以容易连在一起,为了避免过度消化以及吹打。
问题解答2:针对你说的抱团情况,我的处理方法,就是用PBS轻轻吹洗细胞,目的是把贴壁不好的细胞弃掉,你这样重复几次后NCI-H1693细胞;人非小细胞肺癌细状态就能恢复了,还是强调一下,就是消化是不能消化过度,吹打时也要轻柔。另外血清浓度不要太高,这样容易导致细胞加速老化,一般10%就可以了。1200 HBVP 人脑血管周细胞 Human Brain Vascular Pericytes 5 x 10^5 cells/vial
1300 HCPEC 人脉络丛内皮细胞 Human Choroid Plexus Endothelial Cells 5 x 10^5 cells/vial
1310 HCPEpiC 人脉络丛上皮细胞 Human Choroid Plexus Epithelial Cells 5 x 10^5 cells/vial
1320 HCPF 人脉络丛成纤维细胞 Human Choroid Plexus Fibroblasts 5 x 10^5 cells/vial
1400 HMC 人脑膜细胞 Human Meningeal Cells 5 x 10^5 cells/vial
1520 HN 人神经元细胞 Human Neurons 1 x 10^6 cells/vial
1520-10 HN Human Neurons 10 x 10^6 cells/vial
1520-5 HN Human Neurons 5 x 10^6 cells/vial
1530 HCGC 人小脑颗粒细胞 Human Cerebellar Granule Cells 1 x 10^6 cells/vial
1540 HN-h 人海马趾神经元 Human Neurons-hippocampal 1 x 10^6 cells/vial
1550 HCN 人大脑皮层神经元 Human Cortical Neurons 1 x 10^6 cells/vial
1560 HN-bs 人脑干神经元 Human Neurons-brain stem 1 x 10^6 cells
1600 HOPC 人少突胶质前体细胞--贴壁生长 Human Oligodendrocyte Precursor Cells 1 x 10^6 cells/vial
1610 HOPC-os 人少突胶质前体细胞--悬浮生长 Human Oligodendrocyte Precursor Cells-oligospheres 1 x 10^6 cells/vial
1620 HO 人少突胶质细胞 Human Oligodendrocytes 1 x 10^6 cells/vial
1630 HOPC-f 人少突胶质前体细胞--新鲜 Human Oligodendrocyte Precursor Cells-fresh 10 x 10^6 cells/tube
1630-40 HOPC-f40 Human Oligodendrocyte Precursor Cells-fresh 40 x 10^6 cells/tube
1630-80 HOPC-f80 Human Oligodendrocyte Precursor Cells-fresh 80 x 10^6 cells/tube
1630-20 HOPC-f20 Human Oligodendrocyte Precursor Cells-fresh 20 x 10^6 cells/tube
1700 HSC 人雪旺细胞 Human Schwann Cells 5 x 10^5 cells/vial
1710 HPC 人神经束膜细胞 Human Perineurial Cells 5 x 10^5 cells/vial
1800 HA 人星形胶质细胞 Human Astrocytes 1 x 10^6 cells/vial
1810 HA-c 人小脑星形胶质细胞 Human Astrocytes-cerebellar 1 x 10^6 cells/vial
1820 HA-sp 人脊髓星形胶质细胞 Human Astrocytes-spinal cord 1 x 10^6 cells/vial
1830 HA-h 人海马星形胶质细胞 Human Astrocytes-hippocampal 1 x 10^6 cells/vial
1840 HA-bs 人星形胶质细胞--脑干 Human Astrocytes-brain stem 5 x 10^5 cells/vial
1850 HA-mb 人星形胶质细胞 - 中脑 Human Astrocytes - middle brain 5 x 10^5 cells/vial
1870 HRA 人类视网膜星形胶质细胞 Human Retinal Astrocytes 5 x 10^5 cells/vial
1900 HM 人小神经胶质细胞 Human Microglia 5 x 10^5 cells/vial
Dermal Cell System 真皮细胞系统 1200 HBVP 人脑血管周细胞 Human Brain Vascular Pericytes 5 x 10^5 cells/vial
1300 HCPEC 人脉络丛内皮细胞 Human Choroid Plexus Endothelial Cells 5 x 10^5 cells/vial
1310 HCPEpiC 人脉络丛上皮细胞 Human Choroid Plexus Epithelial Cells 5 x 10^5 cells/vial
1320 HCPF 人脉络丛成纤维细胞 Human Choroid Plexus Fibroblasts 5 x 10^5 cells/vial
1400 HMC 人脑膜细胞 Human Meningeal Cells 5 x 10^5 cells/vial
1520 HN 人神经元细胞 Human Neurons 1 x 10^6 cells/vial
1520-10 HN Human Neurons 10 x 10^6 cells/vial
1520-5 HN Human Neurons 5 x 10^6 cells/vial
1530 HCGC 人小脑颗粒细胞 Human Cerebellar Granule Cells 1 x 10^6 cells/vial
1540 HN-h 人海马趾神经元 Human Neurons-hippocampal 1 x 10^6 cells/vial
1550 HCN 人大脑皮层神经元 Human Cortical Neurons 1 x 10^6 cells/vial
1560 HN-bs 人脑干神经元 Human Neurons-brain stem 1 x 10^6 cells
1600 HOPC 人少突胶质前体细胞--贴壁生长 Human Oligodendrocyte Precursor Cells 1 x 10^6 cells/vial
1610 HOPC-os 人少突胶质前体细胞--悬浮生长 Human Oligodendrocyte Precursor Cells-oligospheres 1 x 10^6 cells/vial
1620 HO 人少突胶质细胞 Human Oligodendrocytes 1 x 10^6 cells/vial
1630 HOPC-f 人少突胶质前体细胞--新鲜 Human Oligodendrocyte Precursor Cells-fresh 10 x 10^6 cells/tube
1630-40 HOPC-f40 Human Oligodendrocyte Precursor Cells-fresh 40 x 10^6 cells/tube
1630-80 HOPC-f80 Human Oligodendrocyte Precursor Cells-fresh 80 x 10^6 cells/tube
1630-20 HOPC-f20 Human Oligodendrocyte Precursor Cells-fresh 20 x 10^6 cells/tube
1700 HSC 人雪旺细胞 Human Schwann Cells 5 x 10^5 cells/vial
1710 HPC 人神经束膜细胞 Human Perineurial Cells 5 x 10^5 cells/vial
1800 HA 人星形胶质细胞 Human Astrocytes 1 x 10^6 cells/vial
1810 HA-c 人小脑星形胶质细胞 Human Astrocytes-cerebellar 1 x 10^6 cells/vial
1820 HA-sp 人脊髓星形胶质细胞 Human Astrocytes-spinal cord 1 x 10^6 cells/vial
1830 HA-h 人海马星形胶质细胞 Human Astrocytes-hippocampal 1 x 10^6 cells/vial
1840 HA-bs 人星形胶质细胞--脑干 Human Astrocytes-brain stem 5 x 10^5 cells/vial
1850 HA-mb 人星形胶质细胞 - 中脑 Human Astrocytes - middle brain 5 x 10^5 cells/vial
1870 HRA 人类视网膜星形胶质细胞 Human Retinal Astrocytes 5 x 10^5 cells/vial
1900 HM 人小神经胶质细胞 Human Microglia 5 x 10^5 cells/vial
Dermal Cell System 真皮细胞系统 1200 HBVP 人脑血管周细胞 Human Brain Vascular Pericytes 5 x 10^5 cells/vial
1300 HCPEC 人脉络丛内皮细胞 Human Choroid Plexus Endothelial Cells 5 x 10^5 cells/vial
1310 HCPEpiC 人脉络丛上皮细胞 Human Choroid Plexus Epithelial Cells 5 x 10^5 cells/vial
1320 HCPF 人脉络丛成纤维细胞 Human Choroid Plexus Fibroblasts 5 x 10^5 cells/vial
1400 HMC 人脑膜细胞 Human Meningeal Cells 5 x 10^5 cells/vial
1520 HN 人神经元细胞 Human Neurons 1 x 10^6 cells/vial
1520-10 HN Human Neurons 10 x 10^6 cells/vial
1520-5 HN Human Neurons 5 x 10^6 cells/vial
1530 HCGC 人小脑颗粒细胞 Human Cerebellar Granule Cells 1 x 10^6 cells/vial
1540 HN-h 人海马趾神经元 Human Neurons-hippocampal 1 x 10^6 cells/vial
1550 HCN 人大脑皮层神经元 Human Cortical Neurons 1 x 10^6 cells/vial
1560 HN-bs 人脑干神经元 Human Neurons-brain stem 1 x 10^6 cells
1600 HOPC 人少突胶质前体细胞--贴壁生长 Human Oligodendrocyte Precursor Cells 1 x 10^6 cells/vial
1610 HOPC-os 人少突胶质前体细胞--悬浮生长 Human Oligodendrocyte Precursor Cells-oligospheres 1 x 10^6 cells/vial
1620 HO 人少突胶质细胞 Human Oligodendrocytes 1 x 10^6 cells/vial
1630 HOPC-f 人少突胶质前体细胞--新鲜 Human Oligodendrocyte Precursor Cells-fresh 10 x 10^6 cells/tube
1630-40 HOPC-f40 Human Oligodendrocyte Precursor Cells-fresh 40 x 10^6 cells/tube
1630-80 HOPC-f80 Human Oligodendrocyte Precursor Cells-fresh 80 x 10^6 cells/tube
1630-20 HOPC-f20 Human Oligodendrocyte Precursor Cells-fresh 20 x 10^6 cells/tube
1700 HSC 人雪旺细胞 Human Schwann Cells 5 x 10^5 cells/vial
1710 HPC 人神经束膜细胞 Human Perineurial Cells 5 x 10^5 cells/vial
1800 HA 人星形胶质细胞 Human Astrocytes 1 x 10^6 cells/vial
1810 HA-c 人小脑星形胶质细胞 Human Astrocytes-cerebellar 1 x 10^6 cells/vial
1820 HA-sp 人脊髓星形胶质细胞 Human Astrocytes-spinal cord 1 x 10^6 cells/vial
1830 HA-h 人海马星形胶质细胞 Human Astrocytes-hippocampal 1 x 10^6 cells/vial
1840 HA-bs 人星形胶质细胞--脑干 Human Astrocytes-brain stem 5 x 10^5 cells/vial
1850 HA-mb 人星形胶质细胞 - 中脑 Human Astrocytes - middle brain 5 x 10^5 cells/vial
1870 HRA 人类视网膜星形胶质细胞 Human Retinal Astrocytes 5 x 10^5 cells/vial
1900 HM 人小神经胶质细胞 Human Microglia 5 x 10^5 cells/vial
Dermal Cell System 真皮细胞系统 1200 HBVP 人脑血管周细胞 Human Brain Vascular Pericytes 5 x 10^5 cells/vial
1300 HCPEC 人脉络丛内皮细胞 Human Choroid Plexus Endothelial Cells 5 x 10^5 cells/vial
1310 HCPEpiC 人脉络丛上皮细胞 Human Choroid Plexus Epithelial Cells 5 x 10^5 cells/vial
1320 HCPF 人脉络丛成纤维细胞 Human Choroid Plexus Fibroblasts 5 x 10^5 cells/vial
1400 HMC 人脑膜细胞 Human Meningeal Cells 5 x 10^5 cells/vial
1520 HN 人神经元细胞 Human Neurons 1 x 10^6 cells/vial
1520-10 HN Human Neurons 10 x 10^6 cells/vial
1520-5 HN Human Neurons 5 x 10^6 cells/vial
1530 HCGC 人小脑颗粒细胞 Human Cerebellar Granule Cells 1 x 10^6 cells/vial
1540 HN-h 人海马趾神经元 Human Neurons-hippocampal 1 x 10^6 cells/vial
1550 HCN 人大脑皮层神经元 Human Cortical Neurons 1 x 10^6 cells/vial
1560 HN-bs 人脑干神经元 Human Neurons-brain stem 1 x 10^6 cells
1600 HOPC 人少突胶质前体细胞--贴壁生长 Human Oligodendrocyte Precursor Cells 1 x 10^6 cells/vial
1610 HOPC-os 人少突胶质前体细胞--悬浮生长 Human Oligodendrocyte Precursor Cells-oligospheres 1 x 10^6 cells/vial
1620 HO 人少突胶质细胞 Human Oligodendrocytes 1 x 10^6 cells/vial
1630 HOPC-f 人少突胶质前体细胞--新鲜 Human Oligodendrocyte Precursor Cells-fresh 10 x 10^6 cells/tube
1630-40 HOPC-f40 Human Oligodendrocyte Precursor Cells-fresh 40 x 10^6 cells/tube
1630-80 HOPC-f80 Human Oligodendrocyte Precursor Cells-fresh 80 x 10^6 cells/tube
1630-20 HOPC-f20 Human Oligodendrocyte Precursor Cells-fresh 20 x 10^6 cells/tube
1700 HSC 人雪旺细胞 Human Schwann Cells 5 x 10^5 cells/vial
1710 HPC 人神经束膜细胞 Human Perineurial Cells 5 x 10^5 cells/vial
1800 HA 人星形胶质细胞 Human Astrocytes 1 x 10^6 cells/vial
1810 HA-c 人小脑星形胶质细胞 Human Astrocytes-cerebellar 1 x 10^6 cells/vial
1820 HA-sp 人脊髓星形胶质细胞 Human Astrocytes-spinal cord 1 x 10^6 cells/vial
1830 HA-h 人海马星形胶质细胞 Human Astrocytes-hippocampal 1 x 10^6 cells/vial
1840 HA-bs 人星形胶质细胞--脑干 Human Astrocytes-brain stem 5 x 10^5 cells/vial
1850 HA-mb 人星形胶质细胞 - 中脑 Human Astrocytes - middle brain 5 x 10^5 cells/vial
1870 HRA 人类视网膜星形胶质细胞 Human Retinal Astrocytes 5 x 10^5 cells/vial
1900 HM 人小神经胶质细胞 Human Microglia 5 x 10^5 cells/vial
Dermal Cell System 真皮细胞系统