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简要描述:D2D Elisa试剂盒可检测血清、血浆、细胞培养上清或其它相关生物液体中的含量或者活性表达。进口试剂盒货期短,物流安全迅速,检测数据准确,是您实验*,免费为客户提供全面技术咨询服务及相关产品中英文双版说明书,技术服务:专业,及时,耐心。
产品型号: 96T
所属分类:ELISA试剂盒进口
更新时间:2016-02-19
D2D Elisa试剂盒提供免费与指导,只需来样即可为您免费代测,可测定血清、血浆、细胞培养上清或其它相关生物液体中的含量或者活性表达。
英文简称:D2D ELISA kit
产品包装:试剂盒(含试剂+酶联板+覆膜等)
特异性:可同时检测天然或重组的,且与其他相关蛋白无交叉反应。
包装规格:48T、96T现货供应,质量问题可包换包退,免除您的后顾之忧。
保存条件:2-8℃(频繁使用时);-20℃(较长时间不用时)。
D2D Elisa试剂盒在实验中,发现ELISA显色剂(TMB系统,A,B液等体积混合)的有效期zui多3个月,难以满足试剂盒6个月或以上的保存期要求,请教有何改进措施(添加稳定剂稳定剂有那些)延长ELISA显色剂(TMB系统)的有效期
市售的TMB大多分A,B两液,临用前混匀.单试剂也有售.为提高单试剂的稳定性,降低有机溶剂DMSO,DMF的用量很关键,为了达到此目的,提高TMB的溶解度是一个解决办法,可用柠檬酸-醋酸盐缓冲液(含1%左右的聚乙烯吡咯烷酮,1-10mM H2O2)作为B液,与一定量的A液混合,配制你所需的TMB浓度.试剂中聚乙烯吡咯烷酮的作用是增加TMB在缓冲液中的溶解度.
在ELISA的各种试剂中是否要加防腐剂和抗菌素防腐剂必需加硫柳汞么加叠氮钠行不行
D2D Elisa试剂盒免疫检测试剂中常用的防腐剂有硫柳汞、叠氮钠、抗生素等。叠氮钠是HRP的抑制剂,凡是和HRP接触的试剂,一定不能用;硫柳汞在ELISA试剂中可普遍使用,但因其有毒,较少使用;市面许多试剂盒产品,用抗生素做防腐剂较多,如楼上朋友提到的庆大。当然,如果你的试剂仅在实验室短期使用,其实并不需加防腐剂,常规试剂放4度冰箱,抗体、酶等加甘油分装冻存即可。
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