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简要描述:GPI Elisa试剂盒可检测血清、血浆、细胞培养上清或其它相关生物液体中的含量或者活性表达。进口试剂盒货期短,物流安全迅速,检测数据准确,是您实验*,免费为客户提供全面技术咨询服务及相关产品中英文双版说明书,技术服务:专业,及时,耐心。
产品型号: 96T
所属分类:ELISA试剂盒进口
更新时间:2016-02-18
GPI Elisa试剂盒提供免费与指导,只需来样即可为您免费代测,可测定血清、血浆、细胞培养上清或其它相关生物液体中的含量或者活性表达。
英文简称:GPI ELISA kit
产品包装:试剂盒(含试剂+酶联板+覆膜等)
特异性:可同时检测天然或重组的,且与其他相关蛋白无交叉反应。
包装规格:48T、96T现货供应,质量问题可包换包退,免除您的后顾之忧。
保存条件:2-8℃(频繁使用时);-20℃(较长时间不用时)。
GPI Elisa试剂盒在实验中,发现ELISA显色剂(TMB系统,A,B液等体积混合)的有效期zui多3个月,难以满足试剂盒6个月或以上的保存期要求,请教有何改进措施(添加稳定剂?稳定剂有那些?)延长ELISA显色剂(TMB系统)的有效期?
市售的TMB大多分A,B两液,临用前混匀.单试剂也有售.为提高单试剂的稳定性,降低有机溶剂DMSO,DMF的用量很关键,为了达到此目的,提高TMB的溶解度是一个解决办法,可用柠檬酸-醋酸盐缓冲液(含1%左右的聚乙烯吡咯烷酮,1-10mM H2O2)作为B液,与一定量的A液混合,配制你所需的TMB浓度.试剂中聚乙烯吡咯烷酮的作用是增加TMB在缓冲液中的溶解度.
在ELISA的各种试剂中是否要加防腐剂和抗菌素?防腐剂必需加硫柳汞么?加叠氮钠行不行?
GPI Elisa试剂盒免疫检测试剂中常用的防腐剂有硫柳汞、叠氮钠、抗生素等。叠氮钠是HRP的抑制剂,凡是和HRP接触的试剂,一定不能用;硫柳汞在ELISA试剂中可普遍使用,但因其有毒,较少使用;市面许多试剂盒产品,用抗生素做防腐剂较多,如楼上朋友提到的庆大。当然,如果你的试剂仅在实验室短期使用,其实并不需加防腐剂,常规试剂放4度冰箱,抗体、酶等加甘油分装冻存即可。
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