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组织金属蛋白酶抑制因子2(TIMP2)重组蛋白

更新时间:2016-01-11 点击量:606

组织金属蛋白酶抑制因子2(TIMP2)重组蛋白

Recombinant Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism speciesBos taurus; Bovine (Cattle)
Product No.xyA128Bo01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass25.5kDa
Concentration7.6
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
ResiduesCys27~Pro220 (Accession # P16368) with two N-terminal Tags, His-tag and T7-tag

FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

组织金属蛋白酶抑制因子2(TIMP2)重组蛋白SEQUENCES

The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGM GRGSEF- CSCS PVHPAFCN ADIVIRAKAV NKKEVDSGND IYGNPIKRIQ YEIKQIKMFK GPDQDIEFIY TAPAAAVCGV SLDIGGKKEY LIAGKAEGNG NMHITLCDFI VPWDTLSATQ KKSLNHRYQM GCECKITRCP MIPCYISSPD ECLWMDWVTE KNINGHQAKF FACIKRSDGS CAWYRGAAPP KQEFLDIEDP

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

组织金属蛋白酶抑制因子2(TIMP2)重组蛋白STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

组织金属蛋白酶抑制因子2(TIMP2)重组蛋白About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.